The antimicrobial peptide dermaseptin 01 (DS 01), from the skin secretion of Phyllomedusa hypochondrialis frogs, was immobilized\r\nin nanostructured layered films in conjunction with nickel tetrasulfonated phthalocyanines (NiTsPc), widely used in electronic\r\ndevices, using layer-by-layer technique. The films were used as a biosensor to detect the presence of dopamine (DA), a\r\nneurotransmitter associated with diseases such as Alzheimer�s and Parkinson�s, with detection limits in the order of 10-6 mol L-1.\r\nThe use of DS 01 in LbL film generated selectivity in the detection of DA despite the presence of ascorbic acid found in biological\r\nfluids. This work is the first to report that the antimicrobial peptide and NiTsPc LbL film exhibits electroanalytical activity to\r\nDA oxidation. The selectivity in the detection of DA is a fundamental aspect for the development of electrochemical sensors with\r\npotential applications in the biomedical and pharmaceutical industries.
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